Error message

  • Deprecated function: Methods with the same name as their class will not be constructors in a future version of PHP; views_display has a deprecated constructor in require_once() (line 3138 of /www/webvol10/al/ytucvvu99ijshoe/denator.com/public_html/includes/bootstrap.inc).
  • Deprecated function: Methods with the same name as their class will not be constructors in a future version of PHP; views_many_to_one_helper has a deprecated constructor in require_once() (line 113 of /www/webvol10/al/ytucvvu99ijshoe/denator.com/public_html/sites/all/modules/ctools/ctools.module).
  • Deprecated function: The each() function is deprecated. This message will be suppressed on further calls in _menu_load_objects() (line 569 of /www/webvol10/al/ytucvvu99ijshoe/denator.com/public_html/includes/menu.inc).

LC-MS peptidomics

Recommended protocol

LC-MS peptidomics

Recommended reading

High Identification Rates of Endogenous Neuropeptides from Mouse Brain. Zhang et al., J Proteome Res. May 2012.11(5):2819-27.

Highlights:
- Increased hydrophobic detection range of endogenous peptides due to mixed on column extraction (MOC) using simultaneous elution of both extraction phases
- Improved identification rate by selecting data acquisition type to correctly match instrumentation and sample content

Related products

Used together with the Stabilizor™ system, the Stabilizor™ Peptide Extraction Kit facilitates differentiation and identification of endogenous peptides from degradative peptide fragments.
The kit contains all necessary accessories and solutions to homogenize and extract endogenous peptides from heat-stabilized tissue prior to LC-MS. A fully tested protocol aims to maximize recovery.