Phospho-shotgun proteomics
Recommended protocol
LC-MS Shotgun proteomics for tissue samples
Recommended reading
Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Lundby et al., Nature Communications. June 2012.
Highlights:
- Incorporates heat stabilization into a workflow for standardized screening of tissue phosphoproteomes
- Broadest tissue catalogue of phosphoproteins to date
- 31,480 phosphorylation sites on 7,280 proteins quantified across 14 tissues in rat
- Great variation in phosphorylation sites across tissues