Phospho-shotgun proteomics

Recommended protocol

LC-MS Shotgun proteomics for tissue samples

Recommended reading

Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Lundby et al., Nature Communications. June 2012.

Highlights:
- Incorporates heat stabilization into a workflow  for standardized screening of tissue phosphoproteomes
- Broadest tissue catalogue of phosphoproteins to date
- 31,480 phosphorylation sites on 7,280 proteins quantified across 14 tissues in rat
- Great variation in phosphorylation sites across tissues

Application note, Global phosphorylation analysis