SDS-PAGE & Western blot
Recommeded protocol
Recommended reading
Preserving protein profiles in tissue samples: Different outcomes with and without heat stabilization, M. Ahmed, K. J. Gardiner, Journal of Neuroscience Methods, 2011, 196.
Highlights:
- Inactivitaion of a broad range of protein modifying and processing enzymes, including both phosphatases and kinases.
- Permanent stabilization of the sample over time (0-30 min in RT)
- Shorter handling time from removal of the brain from the scull to inactivation of enzymes for heat-stabilized samples compared to snap frozen or freshly prepared samples.
“Quantitation of phospho-proteins in hippocampal lysates shows that heat stabilized levels remain unchanged while frozen tissue levels are decreased with room temperature incubation.”