Error message

  • Deprecated function: Methods with the same name as their class will not be constructors in a future version of PHP; views_display has a deprecated constructor in require_once() (line 3138 of /www/webvol10/al/ytucvvu99ijshoe/denator.com/public_html/includes/bootstrap.inc).
  • Deprecated function: Methods with the same name as their class will not be constructors in a future version of PHP; views_many_to_one_helper has a deprecated constructor in require_once() (line 113 of /www/webvol10/al/ytucvvu99ijshoe/denator.com/public_html/sites/all/modules/ctools/ctools.module).
  • Deprecated function: The each() function is deprecated. This message will be suppressed on further calls in _menu_load_objects() (line 569 of /www/webvol10/al/ytucvvu99ijshoe/denator.com/public_html/includes/menu.inc).

SDS-PAGE & Western blot

Recommeded protocol

SDS-PAGE & Western blot

Recommended reading

Preserving protein profiles in tissue samples: Different outcomes with and without heat stabilization, M. Ahmed, K. J. Gardiner, Journal of Neuroscience Methods, 2011, 196.

Highlights:
- Inactivitaion of a broad range of protein modifying and processing enzymes, including both phosphatases and kinases.
- Permanent stabilization of the sample over time (0-30 min in RT)
- Shorter handling time from removal of the brain from the scull to inactivation of enzymes for heat-stabilized samples compared to snap frozen or freshly prepared samples.

“Quantitation of phospho-proteins in hippocampal lysates shows that heat stabilized levels remain unchanged while frozen tissue levels are decreased with room temperature incubation.”

Read a personal story from Prof. Katheleen Gardiner on how they have benefited from using the Stabilizor system in their research on Down syndrome.

Application note, Western blot