Publications

Pathogen inactivation

Heat fixation inactivates viral and bacterial pathogens and is compatible with downstream MALDI mass spectrometry tissue imaging. Lisa H Cazares et al., BMC Microbiology 2015, 15:101. Read more 

Lipids

Post-sampling release of free fatty acids - effects of heat stabilization and methods of euthanasia. Jernerén et al., Journal of Pharm and Tox Methods, 2015, 71 13-20. Read more

Simultaneous quantification of sphingolipids in small quantities of liver by LC-MS/MS. Saigusa et al., Mass Spectrometry, 2014, 3. Read more

Endogenous peptides

Analytic framework for peptidomics applied to large-scale neuropeptide identification. A. Secher et al., Nature Communications 7. May, 2016. Read more 

Uncovering eects of ex-vivo protease activity during proteomics and peptidomics sample extraction in rat brain tissue by oxygen-18 labelling”. C. Stingl et al., Journal of Proteome Research. May, 2014. Read more

Identification and quantification of neuropeptides in naïve mouse spinal cord using mass spectrometry reveals [des-Ser1]-cerebellin as a novel modulator of nociception. J. Sue et al., Journal of Neurochemistry. May, 2014. Read more

Abnormal structure-specific peptide transmission and processing in a primate model of Parkinson's disease and L-DOPA-induced dyskinesia. M. Bourdenx et al., Neurobiology of Disease. Feb. 2014 Vol. 62,  307-312. Read more

Improving molecular distribution analysis. M. Borén. GEN, Tutorial. July 2013. Read more

Neurotoxin-induced neuropeptide pertubations in striatum of neonatal rats. Karlsson et al.,Journal of Proteome Research. Mars 2013. Read more

Chronic nicotine treatment impacts the regulation of opioid and non-opioid peptides in the rat striatum. Petruzziello et al.,Molecular and Cellular Proteomics. February 2013. Read more

Conductive carbon tape used for support and mounting of both whole animal and fragile heat-treated tissue sections for MALDI MS imaging and quantitation. Goodwin et al., Journal of Proteomics. Aug 2012. Vol. 75, issue 16, 4912-4920. Read more
Keywords: Peptides, MALDI MS imaging, rat tissues

High Identification Rates of Endogenous Neuropeptides from Mouse Brain. Zhang et al., J Proteome Res. May 2012. 11(5):2819-27. Read more
Keywords: Neuropeptidomics, mass spectrometry, mouse brain

Probing Neuropeptide Signaling at the Organ and Cellular Domains via Imaging Mass Spectrometry. Ye et al., J of Proteomics. Aug 2012, vol. 75, issue 16, 5014-5026. Read more
Keywords: Imaging mass spectrometry, neuropeptides, neuroendocrine tissues

Extensive Characterization of Tupaia belangeri Neuropeptidome Using an Integrated Mass Spectrometric Approach. Petruzziello et al., J. Proteome Res. 2012, 11 (2), pp 886–896. Read more
Keywords: Tree shrew brain (Tupaia belangeri), mass spectrometry

Neuropeptide profiling of the bovine hypothalamus: Thermal stabilization is an effective tool in inhibiting post-mortem degradation. Colgrave et al., Proteomics 2011, 11, 1264-1276. Read more
Keywords: Bovine brain, mass spectrometry

Stopping the clock on proteomic degradation by heat-treatment at the point of tissue excision. Richard J. A. Goodwin et al. Proteomics, Volume 10, Issue 9, pages 1751-1761, No. 9 May 2010. Read more
Keywords: Mouse brain, MALDI-imaging, 2D-GE, mass spectrometry

Neuropeptidomic analysis of the embryonic Japanese quail diencephalon, Scholz, B., H. Alm, et al., BMC Dev Biol 10: 30. Read more
Keywords: Japanese quail brain, mass spectrometry

Impact of temperature dependent sampling procedures in proteomics and peptidomics - A characterization of the liver and pancreas post mortem degradome, B Scholz et al. Molecular and Cellular Proteomics, M900229-MCP200, January 28, 2010. Read more
Keywords: Mouse liver and pancreas, 2D-GE, mass spectrometry

Development and evaluation of normalization methods for label-free relative quantification of endogenous peptides. K. Kultima, Molecular and Cellular Proteomics, October 1, 2009, 8, 2285-2295. Read more
Keywords: Mouse -, rat - and Japanese quail brain, mass spectrometry

A Quantitative Peptidomic Analysis of Peptides Related to the Endogenous Opioid and Tachykinin Systems in Nucleus Accumbens of Rats Following Naloxone-Precipitated Morphine Withdrawal U. Rossbach et al. J. Proteome Res. 2009 Oct;9(20):4616-26. Read more
Keywords: Rat brain, mass spectrometry

Heat Stabilization of the Tissue Proteome: A New Technology for Improved Proteomics, M. Svensson et al. J. Proteome Res., 2009, 8 (2), pp 974-981. Read more
Keywords: Mouse brain, mass spectrometry, Western Blot

Protein and peptides in pictures: imaging with MALDI mass spectrometry, RJ. Goodwin et al. Proteomics., 2008 Sep;8(18):3785-800. Read more
Keywords: Mammalian tissue, MALDI-imaging

The significance of biochemical and molecular sample integrity in brain proteomics and peptidomics: stathmin 2-20 and peptides as sample quality indicators. K. Sköld et al. Proteomics. 2007 Dec;7(24):4445-56. Read more
Keywords: Mouse brain, 2D-DIGE, Western blot, mass spectrometry

Neuropeptidomics: MS Applied to the Discovery of Novel Peptides from the Brain M. Svensson et al. Anal. Chem., 2007, 79 (1), pp 14-21. Read more
Keywords: Rat and mouse brain, mass spectrometry, MALDI-imaging

Neuropeptidomics: expanding proteomics downwards. M. Svensson et al. Biochem Soc Trans 2007, 35, (Pt 3), pp 588-93. Read more
Keywords: Mouse brain, mass spectrometry, MALDI-imaging

Phosphoproteins & other PTMs

Combined effect of tissue stabilization and protein extraction methods on phosphoprotein analysis. Kofanova et al., Biopreservation and Biobanking. May 2013. Read more

Protein profiles in Tc1 mice implicate novel pathway perturbations in the Down syndrome brain. Ahmed et al., Human Molecular Genetics. January 2013. Read more
Keywords: Down syndrome, mouse model, phospho-proteins, RPPA

Expression of trisomic proteins in Down syndrome model systems. Spellman et al., Gene. October 2012. In press, accepted manuscript. Read more
Keywords: Down syndrome, quantitative Western Blot, mouse brain

Proteomic Analysis of Lysine Acetylation Sites in Rat Tissues Reveals Organ Specificity and Subcellular Patters. Lundby et al., Cell reports. August 2012. Vol. 2, issue 2, 419-431. Read more
Keywords: Acetylations, proteomics, mass spectrometry, rat tissues

Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Lundby et al., Nature Communications. June 2012. Read more
Keywords: Phosphorylations, phosphoproteomics, quantitative mass spectrometry, rat tissues

Loss of correlations among proteins in brains of the Ts65Dn mouse model of Down syndrome. Ahmed et al., J. of Proteome research. 2012, 11 (2), pp 1251–1263. Read more
Keywords: Down syndrome, Trisomy 21, MAP kinase, Reverse Phase Protein Array (RPPA), phospho-proteins

Effect of Thaw Temperatures in Reducing Enzyme Activity in Human Thyroid Tissues. Kokkat et al., Biopreservation and Biobanking. 2011, 9(4): 349-354. Read more
Keywords: Human thyroid tissue, phosphatase activity

Thermal stabilization of tissues and the preservation of protein phosphorylation states for two-dimensional gel electrophoresis. Smejkal et al., Electrophoresis 2011, Volume 32, pages 2206–2215. Read more
Keywords: Mouse brain, 2D-GE, mass spectrometry

Clinical application for the preservation of phospho-proteins through in-situ tissue stabilization, CB Rountree et al, Proteome Science, 2010; 8: 61. Read more
Keywords: Mouse brain, lung and liver, 2D-GE, Western Blot

Preserving protein profiles in tissue samples: Differing outcomes with and without heat stabilization, M Ahmed et al, Journal of Neuroscience Methods. 2011 Mar 15;196(1):99-106. Read more
Keywords: Mouse brain, Western Blot, phospho-proteins

Heat Stabilization of the Tissue Proteome: A New Technology for Improved Proteomics, M. Svensson et al. J. Proteome Res., 2009, 8 (2), pp 974-981. Read more
Keywords: Mouse brain, mass spectrometry, Western Blot

Small molecules

Improving molecular distribution analysis. M. Borén. GEN, Tutorial. July 2013. Read more

Localisation of adenine nucleotides in heat-stabilised mouse brains using ion mobility enabled MALDI Imaging. Blatherwick et al., International Journal of Mass Spectrometry. February 2013. In press. Read more
Keywords: Adenine nucleotides ATP, ADP and AMP, mouse brain, MALDI Imaging, metabolic stress

Heat stabilization of blood spot samples for determination of metabolically unstable drug compounds. Blessborn et al., Bioanalysis. (5) 1, 2013 pp 31-39. Read more
Keywords: DBS, blood, small molecules, LC-MS 

Conductive carbon tape used for support and mounting of both whole animal and fragile heat-treated tissue sections for MALDI MS imaging and quantitation. Goodwin et al., Journal of Proteomics. Aug 2012. Vol. 75, issue 16, 4912-4920. Read more
Keywords: Peptides, MALDI MS imaging, rat tissues

Publications & reviews on sample preparation

Uncovering effects of ex-vivo protease activity during proteomics and peptidomics sample extraction in rat brain tissue by oxygen-18 labelling. C. Stingl et al., Journal of Proteome Research. May 2014. Read more

11 Golden rules of working with proteomic samples. M. Borén. GEN, Expert tips. August 2013. Read more

The impact of biosampling procedures on molecular data interpretation. Skold et al., Molecular & cellular proteomics. February 2013. In press. Read more

Combined effect of tissue stabilization and protein extraction methods on phosphoprotein analysis. Kofanova et al., Biopreservation and Biobanking. May 2013. Read more

Eliminating biological change after excision. M Söderquist., GEN Jan. 2013. Tutorial. Read more

Is phosphoproteomics ready for clinical research? Iliuk et al., Clinica Chimica Acta. May 2013. Read more

MALDI MS imaging as a tool for biomarker discovery: Methodological challenges in a clinical setting. Minerva et al., Proteomics- Clinical applications. December 2012. Read more

A vision for better health: Mass spectrometry imaging for clinical diagnostics. Ye et al., Clinica Chimica Acta. October 2012. In press, uncorrected proof. Read more

Sample Preparation Techniques for the Untargeted LC-MS-Based Discovery of Peptides in Complex Biological Matrices. Finoulst et al., J. Biomedicine and Biotechnology. September 2011. Read more
Keywords: Peptidomics, LC-MS, sample preparation

Sample Preparation in Biological Mass Spectrometry, Alexander R. Ivanov, Alexander V. Lazarev. Read more

Biomarkers of disease and post-mortem changes-heat stabilization, a necessary tool for measurement of protein regulation. Kultima et al., Journal of Proteomics, 2011, Volume 75, Issue 1, 145–159. Read more
Keywords: Degradation, post-sampling change, cancer-specific biomarkers

Benefits of heat-treatment to the protease packe neutrophil for proteome analysis: Halting protein degradation. Kennedy et al., Proteomics, 2011, 11, 2560-2564. Read more
Keywords: Neutrophiles, 2D-GE, mass spectrometry

The use of neuroproteomics in drug abuse research, M Lull et al, Drug and Alcohol Dependence, Volume 107, Issue 1, 1 February 2010, Pages 11-22. Read more

Banking of clinical samples for proteomic biomarker studies: A consideration of logistical issues with a focus on pre-analytical variation, Jackson, D.H., and Banks, R.E., Proteomics - Clinical Applications 2010 4:3, 250-270. Read more

A report on the ESF workshop on quality control in proteomics, L Martens. Mol. BioSyst., 2010, 6, 935-938. Read more

Assessing the use of thermal treatment to preserve the intact proteomes of post-mortem heart and brain tissue, AA. Robinson et al. Proteomics., Article 2009 Oct;9(19):4433-44. Read more
Keywords: Mouse brain and heart, 2D-GE, mass spectrometry

Preserving the yeast proteome from sample degradation, J. Grassl et al. Proteomics., Proteomics. 2009 Oct;9(20):4616-26. Read more
Keywords: Yeast, 2D-GE, mass spectrometry

Heat Stabilization of the Tissue Proteome: A New Technology for Improved Proteomics, M. Svensson et al. J. Proteome Res., 2009, 8 (2), pp 974-981. Read more
Keywords: Mouse brain, mass spectrometry, Western Blot

Sample preparation issues for tissue imaging by imaging MS, Kaletas, B. K., I. M. van der Wiel, et al. Proteomics 9(10): 2622-33. Read more
Keywords: Human cerebellum tissue, MALDI-imaging