The principles of heat stabilization
Heat stabilization is carried out using the Stabilizor™ system together with Maintainor Tissue cards. It is a rapid, straight-forward, automated procedure that delivers consistent, high quality and reproducible results.
Heat stabilization uses rapid conductive heating, under controlled pressure, to generate a fast, homogenous and irreversible thermal denaturation of proteins. This results in complete and permanent elimination of all enzymatic activity that would otherwise cause further biological changes to the tissue sample ex vivo.
The Stabilizor technology builds on the principle of thermal denaturation to ensure inactivation of proteins and enzymes.
In their active, native form, proteins are dependent on their 3D-fold. During heat stabilization, the proteins unfold into inactive, random configurations. After heat stabilization, proteins assume a new denatured and stable fold. This results in the prevention of further enzymatic change of the sample.
The fully automated procedure ensures consistent and reproducible treatment of samples. Sample size is determined using a highly accurate laser. Size, together with information about the physical state of the sample, for example fresh or frozen, is fed into algorithms of pre-programmed method templates. These algorithms determine the exact amount of energy required to ensure complete denaturation.