Why use heat stabilization?
Understanding the role of proteins, peptides and small molecules in normal and diseased tissue is crucial to defining their potential use as drugs, drug targets or disease biomarkers. Yet biological changes begin the moment tissue is removed from its native environment. Dramatic alterations at the molecular level occur within seconds. Vital information may be lost or distorted, leading to inter-sample variation, risk of incorrect data interpretation and potentially misleading conclusions.
- Control mechanisms are disrupted; phosphorylation states are altered and proteins begin to degrade
- Changed metabolism; catabolic fragmentation of large molecules (such as ATP) occurs in order to release energy
- Represents a major analytical challenge when trying to elucidate in vivo biological mechanisms or identity or monitor potential biomarkers.
Heat stabilization overcomes the drawbacks of conventional tissue sample preservation techniques, such as snap-freezing followed by inhibitors. In heat stabilized tissue, biological change is stopped immediately and permanently.
Prevent biological change and ensure accurate downstream results
If you are studying any kind of molecule susceptible to biological change post-sampling, tissue sample handling is a critical success factor. Using the Stabilizor™ system preserves molecular profiles as close as possible to their in vivo state. This improves the quality and accuracy of your analytical results.
- Detect more, novel endogenous peptides
- Enable accurate analysis of lipids and other metabolites
- Preserve phosphorylation states
- Enable proteomic analysis of infectious samples
- Enable both proteomic and genomic analyses
- Improve detection of small molecules
Simplify and standardize sampling procedures
The Stabilizor system is extremely easy to use, allowing sample handling to be carried out by anyone. Full automation together with registration of sample details, including time since excision, help you to standardize, monitor and control sampling procedures, and improve the consistency and reproducibility of your results.